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Recombinant Cynomolgus GFRAL/GFR alpha-like Protein,His-Avi Tag
- 品牌:翌圣生物
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翌圣生物科技(上海)股份有限公司
更新時間:2025-01-17 11:16:41
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入駐年限第2年
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詳細介紹
性能參數(shù)
分子別名(Synonyms)
GRAL; GFRAL;GFR alpha-like; GFR alpha like
表達區(qū)間及表達系統(tǒng)(Source)
Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu351.[Accession | XP_015304775.2]
分子量大小(Molecular Weight)
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
內(nèi)毒素(Endotoxin)
Less than 1EU per μg by the LAL method.
純度(Purity)
> 95% as determined by SDS-PAGE and HPLC.
活性(Activity)
ELISA Data: Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
制劑(Formulation)
Lyophilized from 0.22μm filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
重構(gòu)方法(Reconstitution)
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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