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Recombinant Cynomolgus IgE Protein,His-Avi Tag
- 品牌:翌圣生物
- 產地:
- 供應商報價:面議
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翌圣生物科技(上海)股份有限公司
更新時間:2024-12-24 09:03:45
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詳細介紹
性能參數
分子別名(Synonyms)
IgE;Ig epsilon chain C region;
表達區間及表達系統(Source)
Cynomolgus IgE Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Lys208-Lys429.[Accession | G8F4W7]
分子量大小(Molecular Weight)
The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on SDS-PAGE result.
內毒素(Endotoxin)
Less than 1EU per μg by the LAL method.
純度(Purity)
> 95% as determined by SDS-PAGE and HPLC.
活性(Activity)
ELISA Data: Immobilized Cynomolgus IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
制劑(Formulation)
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構方法(Reconstitution)
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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