a级片免费网站_成人黄色一区_日本无遮挡在线观看_在线视频免费观看国产_日韩视频中文字幕视频一区_日韩免费视频一区

儀器網（yiqi.com）歡迎您！

| 注冊 登錄
網站首頁-資訊-專題- 微頭條-話題-產品- 品牌庫-搜索-供應商- 展會-招標-采購- 社區-知識-技術-資料庫-方案-產品庫- 視頻

產品中心

當前位置：儀器網>產品中心> 廣州安邦生物科技有限公司>生物試劑>抗體>Human HMGB1 recombinant protein (Active) (His-tagg
收藏  

Human HMGB1 recombinant protein (Active) (His-tagg

立即掃碼咨詢

聯系方式：400-822-6768

聯系我們時請說明在儀器網（www.shangjinews.cn）上看到的!

掃    碼    分   享
為您推薦

產品特點

HMGB1 是一種多功能蛋白，在炎癥與腫瘤均扮演重要角色。HMGB1 可做為損傷相關分子模式 (DAMP) 分子誘發炎癥；然而過量 HMGB1 所誘發的高強度炎癥反而會導致機體組織損傷。另外，腫瘤細胞分泌的 HMGB1 可促進血管生成與免疫抑制，從而推進腫瘤進展。

詳細介紹

概述

產品描述E. coli expressed, His-tagged (C-ter) Active Human HMGB1 recombinant protein.
反應物種Hu, Ms
應用FuncSt, SDS-PAGE
靶點名稱HMGB1
物種Human
A.A. 序列MGKGD PKKPR GKMSS YAFFV QTCRE EHKKK HPDAS VNFSE FSKKC SERWK TMSAK EKGKF EDMAK ADKAR YEREM KTYIP PKGET KKKFK DPNAP KRPPS AFFLF CSEYR PKIKG EHPGL SIGDV AKKLG EMWNN TAADD KQPYE KKAAK LKEKY EKDIA AYRAK GKPDA AKKGV VKAEK SKKKK EEEED EEDEE DEEEE EDEED EDEEE DDDDE with polyhistidine tag at the C-terminus.
表達系統E. coli
活動Active
活動說明Measure by its ability to induce TNF alpha in Raw264.7 cells.
for this effect is < 10 μg/ml. This protein also induces TNF alpha releasing from human A549 cultured cells based on customer feedback.
別名HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

屬性

形式Powder
純化Ni-NTA chromatography.
純化說明Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
純度> 98% (by SDS-PAGE)
緩沖液PBS (pH 8.0)
重建It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放說明Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事項For laboratory research only, not for drug, diagnostic or other use.

生物信息

數據庫連接

 GeneID: 15289 Mouse HMGB1

 GeneID: 3146 Human HMGB1

 Swiss-port # P09429 Human High mobility group protein B1

 Swiss-port # P63158 Mouse High mobility group protein B1

基因名稱HMGB1
全名high mobility group box 1
背景介紹HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
細胞定位Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. Note=In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus. Translocates from the nucleus to the cytoplasm upon autophagy stimulation. Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes. [UniProt]
產品亮點Related products:
HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
Total solution for HMGB1 research
HMGB1 in inflammation
Inflammatory Cytokines
HMGB1 ELISA Kit for your research
Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit
New HMGB1 neutralizing antibody is released
Detecting exosomal HMGB1 for ICD research
Related poster download:
HMGB1 Pathway.pdf
預測分子量25 kDa
翻譯后修飾Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).

Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).

Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).

Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).

In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt]


廠商推薦產品

在線留言

換一張？
取消
主站蜘蛛池模板： 国产精品激情欧美可乐视频 | 亚洲黄网在线观看 | 玉女阁福利视频导航 | 久草国产视频 | 他掀开裙子舌头伸进去在线观看 | 女人下边被添全过程A片图片 | 国产一级免费在线观看 | 人人澡人人透人人爽 | 日本一区二区三区久久久 | 亚洲精品一区二区成人 | 国产二区视频在线观看 | 国产精品麻豆va在线播放 | 超碰天天操 | 国产免费又黄又爽又刺激蜜月al | 欧美顶级少妇做爰hd | 中文字幕理论片 | 一区二区三区毛片免费 | 国产一级毛片国语视频 | 日本精品久久久一区二区三区 | 精品综合久久久久久98 | 少妇福利视频 | 91国内视频在线 | 毛片视频网址 | 欧美xxxx日本和非洲 | 再深点灬舒服灬太大了添少妇字幕 | 四川小少妇BBAABBAA | 亚洲国产精品久久久久秋霞蜜臀 | 少妇一级淫片高潮流水在线观看 | 日本成人不卡 | 黑人粗大猛烈进出高潮视频 | 96久久免费视频 | 91精品国产足浴勾搭女居士 | 久草播放| 久久国产午夜精品理论片最新版本 | a天堂最新地址 | 国内一区二区 | 国产亚洲精品美女久久久M 亚洲第一在线 | 刘亦菲毛片一区二区三区 | 搜索毛片| 最新国模无码国产在线视频 | 国产剧情一区二区在线观看 |