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Recombinant Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein,His-Avi Tag

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詳細介紹

性能參數

分子別名（Synonyms）	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
表達區間及表達系統（Source）	Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.[Accession \| AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK]
分子量大小（Molecular Weight）	The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on SDS-PAGE result.
內毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
純度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.32μg/ml determined by ELISA.
制劑（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

儲存條件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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