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Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein,His-Avi Tag

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詳細介紹

性能參數

分子別名（Synonyms）	beta3; Integrin alpha V beta 3;αVβ3; ITGAV&ITGB3
表達區間及表達系統（Source）	Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail.[Accession \| P06756-1(ITGAV)&P05106-1(ITGB3)]
分子量大小（Molecular Weight）	The protein has a predicted MW of 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on SDS-PAGE result.
內毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
純度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
制劑（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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