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Recombinant Biotinylated Human FcRn Protein,His-Avi Tag

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詳細介紹

 

性能參數

分子別名(Synonyms)

FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M

表達區間及表達系統(Source)

Biotinylated Human FcRn Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala24-Ser297(FCGRT)&Ile21-Met119(B2M).[Accession | P55899(FCGRT)&P61769((B2M)]

分子量大小(Molecular Weight)

The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on SDS-PAGE result.

內毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

純度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

SPR Data:Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay .

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

儲存條件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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