表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu20-Pro365.[Accession | P08887-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data:Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 1.04μg/ml determined by ELISA.  

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.