分子別名（Synonyms）

RING-type E3 ubiquitin transferase RNF43;RING finger protein 43;E3 ubiquitin-protein ligase RNF43;

表達區間及表達系統（Source）

Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly24-Tyr197.[Accession | Q5NCP0]

分子量大小（Molecular Weight）

The protein has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.40μg/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.