分子別名（Synonyms）

CD274; PD-L1B7 homolog 1; PD-L1; B7H1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Phe19-Thr238.[Accession | NP_068693]

分子量大小（Molecular Weight）

The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

SPR Data: Mouse PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse PD-L1, His Tag with an affinity constant of 3.06 μM as determined in SPR assay .

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.