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Recombinant Biotinylated Human IL-21 Protein,His-Avi Tag

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詳細(xì)介紹

 

性能參數(shù)

分子別名(Synonyms)

Za11; IL-21;Interleukin-21;

表達區(qū)間及表達系統(tǒng)(Source)

Biotinylated Human IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus. It contains Gln32-Ser162.[Accession | Q9HBE4-1]

分子量大?。∕olecular Weight)

The protein has a predicted MW of 18.30 kDa same as SDS-PAGE result.

內(nèi)毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

純度(Purity)

> 95% as determined by SDS-PAGE

活性(Activity)

ELISA Data: Immobilized Human IL-21R, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 2.5ng/ml determined by ELISA.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in 20mM NaAc, 500mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 500mM NaCl (pH 5.5).

 

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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