分子別名（Synonyms）

NAG-1; MIC-1;GDF-15; PLAB

表達區(qū)間及表達系統(tǒng)（Source）

Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser189-Ala303.[Accession | Q9Z0J6]

分子量大小（Molecular Weight）

The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE

活性（Activity）

ELISA Data: Immobilized Rat GDF-15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA .Immobilized Rat GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95μg/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).