INTRODUCTION This technical note describes a simple plumbing scheme that allows optimization for both mass-directed collection of fractions, and subsequent automated reinjection of those fractions using off-the-shelf Gilson instrumentation. The dual system (prep and analytical) utilized a diode array detector and a VERITY? 1900 MS Detector (Figure 1 ). A 2-position, 10-port VALVEMATE? II was employed to switch the pump to the injector, the column to the detector, and to maintain the UV detector,
INTRODUCTION
This technical note describes a simple plumbing scheme that allows optimization for both mass-directed collection of fractions, and subsequent automated reinjection of those fractions using off-the-shelf Gilson instrumentation. The dual system (prep and analytical) utilized a diode array detector and a VERITY? 1900 MS Detector (Figure 1 ). A 2-position, 10-port VALVEMATE? II was employed to switch the pump to the injector, the column to the detector, and to maintain the UV detector,[詳細]
APPLICATION BENEFITS
The necessity to perform purification with subsequent analysis via HPLC to determine the composition of collected fractions consumes scientists’ time and money. Utilization of multiple instruments often requires the user to learn multiple software applications. This causes scientists to spend more time learning software functionalities, and less time applying their knowledge to scientific discoveries.[詳細]
INTRODUCTION
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY? 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors. [詳細]
INTRODUCTION
Liquid chromatography coupled with mass spectrometry (LCMS) is widely used in both analytical and preparative chemistry applications. In this process, compounds in a complex mixture are separated based on their size or chemical properties using LC, and the mass signal from the MS detector is used to confirm compound identity and trigger fraction collection. [詳細]
INTRODUCTION
A general liquid hand領 application is to use a liquid handler as an autosampler for a spectrophotometer. The traditional manual method for fil領 a flow-through cuvette is to use a peristaltic pump (a sipper) to draw the sample through the cuvette. This can easily be replicated using a Gilson GX liquid handler. [詳細]
INTRODUCTION
In laboratories specializing in milk analysis, there are two methods where Gilson could help with a semi- or full automation of the complete protocols. In comparison to the manual procedure, automation provides more robust and reproducible results and sample traceability. [詳細]
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